In Spain, all the goals came in the opening 32 minutes as Frankfurt won 2-1 at Real Betis.
Xavi Hernandez's Barca will continue their European campaign in the second-tier competition, with the winner of their tie with Napoli advancing to the last 16.
Diego Simeone's Atletico needed to beat Porto in Portugal to advance to the knockout phase and they duly triumphed 3-1 in a game which featured three red cards.
A goalless draw at home to Benfica left Barca with their destiny in their own hands, but with a much trickier final game away to Bayern Munich
Champions League: ‘I have full confidence in my team,’ says Simeone as Atletico face Porto in must-win clash
Defeat would see Atletico Madrid miss out on the knockout stage for only the second time during Diego Simeone's near decade-long tenure.
Sporting, who have advanced to the round of 16 in the Champions League, and Porto, who are second in their group, could be excluded the next time they qualify for the Champions League, Europa League or Europa Conference League in the next three seasons.
Liverpool midfielders Fabinho and Thiago Alcantara are back in contention for the Champions League visit of Atlético Madrid after recovering from injuries.
Liverpool head to Atletico Madrid seeking a third straight win in the group and likely to welcome back Brazil internationals Alisson Becker and Fabinho after their late returns from national team duty.
Champions League: Lionel Messi opens account as PSG beat Manchester City; Sheriff Tiraspol shock Real Madrid
In Group D, Sheriff, playing in just their second ever game in the group stage of the competition, upset record 13-time winners Real Madrid 2-1 in the Spanish capital.
Here's all you need to know about the Matchday 2 of UEFA Champions League on Tuesday.
Istanbul was scheduled to host the Champions League final but that became impossible when the UK government put Turkey on its red list.
Villas-Boas has been out of a job since leaving Marseille in acrimonious circumstances earlier this year.
Champions League: Ahead of Manchester City-Chelsea final, Portugal lifts restrictions to allow British tourists entry
Lisbon's move came after London had placed Portugal on its 'green list' of countries to visit without having to self-isolate on return home.
The 29 May showpiece was moved from Istanbul to the 50,000-capacity Estádio do Dragão due to England imposing tougher pandemic travel restrictions on Turkey that would have prevented fans flying in.
UEFA is hoping for more than 10,000 people to be at the Porto stadium after having to move the final from Istanbul to Portugal for a second successive year.
Tuchel's side will play Real Madrid or Liverpool for a place in the final after producing a masterclass in game management to win 2-1 on aggregate in Seville.
Champions League: Bayern Munich, Porto, Liverpool face stiff odds in comeback bid, Man City look to exploit Dortmund's defence
In the Champions League second legs, Bayern Munich, Porto, Liverpool, Borussia Dortmund come from behind against PSG, Chelsea, Real Madrid and Manchester City
Mount's slick turn and shot in the first half would have been painful enough for Porto, who were the better side for large spells in Seville but repeatedly lacked the precision to take advantage.
Chelsea boss Thomas Tuchel hails Antonio Rudiger, Kepa Arrizabalaga for making amends following bust-up
The manager confirmed that Germany defender Rudiger and Spain goalkeeper Kepa had clashed — but insisted the matter was finished.
Champions League: Liverpool out for reconciliation against Real Madrid, Manchester City hope to end quarter-final jinx
Real Madrid vs Liverpool, Man City vs Dortmund, Bayern vs PSG, Porto vs Chelsea; there are the matches we'll witness play out over Tuesday and Wednesday, with signature capriciousness of the traditional Champions league quarter-finals.