In Naples, Lazio's five-match winning streak ended but they are still not out of the Champions League running with a game in hand.
Gasperini won the Panchina d'Oro (the Golden Bench) following a vote of his coaching colleagues in the Italian Coaches' Association.
Serie A: Romelu Lukaku scores inside first minute as Inter Milan pull seven points clear; AC Milan beat Roma
Atalanta shook off their Champions League defeat to Real Madrid midweek with a 2-0 win at Sampdoria to move up to fourth.
Champions League: Ferland Mendy's late strike helps Real Madrid beat Atalanta in Round of 16 first leg
Madrid have the advantage going into the second leg having missed out on the quarter-finals for the last two seasons.
There is a vast gap between 13-time European champion Real Madrid and Atalanta, who are making only their second appearance in the competition.
Napoli goalkeeper David Ospina proved decisive on the night pulling off key saves as Atalanta came closest to breaking the deadlock in the Diego Armando Maradona Stadium.
Serie A: Atalanta captain Papu Gomez fuels talk of transfer after reported bust-up with coach Gian Piero Gasperini
The 32-year-old reportedly had a bust-up with coach Gian Piero Gasperini at half-time during the Bergamo club's Champions League draw against Midtjylland two weeks ago, and has been left out of their last two league games.
Serie A: Inter Milan left furious by contentious VAR decision during draw against Parma; Atalanta beat Crotone
Inter had a strong penalty appeal when Botond Balogh hauled down Ivan Perisic in the second half, with Conte skipping his press conference, apparently in protest at the decision.
Atalanta took the lead at the Stadium of Light thanks to Mario Pašalić's first-half goal, but Marquinhos equalised in the 90th and Eric Choupo-Moting scored the winner in the third minute of stoppage time.
De Roon reiterated a promise he had made a few months ago after being asked by an Atalanta fan about what he would do if his team won the European title. The Dutch player said he would throw a party in Bergamo's central square and everyone would be invited.
Serie A: Only way to avoid handballs is to 'cut off players' arms', says angry Atalanta coach Gian Piero Gasperini
Gasperini saw his side give away two penalties, both for handball, which allowed Serie A leaders Juventus twice to fight back from behind.
Serie A: Ronaldo's penalties help Juventus salvage draw against Atalanta; Sassuolo beat Lazio with stoppage time goal
Juventus needed two penalties from Cristiano Ronaldo - including a 90th-minute equalizer - to prevent Atalanta from winning its 12th straight match in all competitions.
Atalanta's 2-0 win halted a run of five straight league wins for Napoli, which also won the Italian Cup last month.
Valencia criticises Atalanta coach Gian Piero Gasperini for taking charge in Champions League game despite showing COVID-19 symptoms
Valencia have criticised Atalanta coach Gian Piero Gasperini for taking charge of his side’s Champions League match against the Spanish side in March despite suffering from symptoms of the novel coronavirus.
Gasperini revealed he first started feeling sick on 9 March, a day before Atalanta played at Valencia in the second leg of the Champions League round of 16.
Serie A: Bullish Atalanta 'believe' they can beat table-toppers Inter Milan, says coach Gian Piero Gasperini
Atalanta’s domestic form dropped while they were distracted by their first-ever Champions League appearance but, with only the league to concentrate on until February, they have begun to climb again and are back up to fifth
Serie A: Cristiano Ronaldo crowned player of the year, Atalanta's Gian Piero Gasperini wins coach award
Ronaldo was at the Gran Gala del Calcio in Milan to collect his prize, instead of attending the Ballon d’Or ceremony in Paris where he was third in the voting for best player in the world.
Luis Muriel hit a hat-trick as Atalanta put their midweek Champions League humiliation behind them with a crushing 7-1 win over 10-man Udinese on Sunday that moved them to within three points of Serie A leaders Juventus.
Cristiano Ronaldo and Miralem Pjanic sealed a 2-1 win for Juventus over Bologna to consolidate the top spot in Serie A on Saturday.
Immobile scored from the spot on 69 minutes and less than 60 seconds later set up Joaquin Correa for the second, before capping the comeback two minutes into injury time with his ninth goal in eight league games this term.