Argentine forward Adolfo Gaich scored the only goal after 69 minutes following a bad pass across the penalty area from Juventus midfielder Arthur.
Juve sit three points behind second-placed AC Milan, who host Udinese on Wednesday, with Inter Milan four points further clear ahead of their match at Parma on Thursday.
Serie A: Juventus forward Paulo Dybala expected to be out for 20 days after picking up knee ligament injury
In a statement, Juventus said that Argentina forward Dybala had undergone examinations at the club's medical centre which revealed an injury to "the medial collateral ligament of the left knee with recovery times of approximately 15/20 days".
AC Milan maintained their one-point advantage over city rivals Inter Milan who missed a chance to go top with a 2-1 loss at Sampdoria.
Serie A: Zlatan Ibrahimovic scores brace as AC Milan clinch derby win against Inter; Napoli thrash Atalanta
Milan won their opening four matches for the first time since the 1995-1996 season when they won the title under Fabio Capello.
Euro 2020 Qualifiers: Spain round off excellent campaign with 5-0 dismantling of Romania; Italy romp to massive 9-1 win over Armenia
Fabian Ruiz put Spain in front after eight minutes while forward Gerard Moreno struck twice and then forced an own goal from Romania’s Adrian Rus before the break, with Mikel Oyarzabal hitting the fifth in second-half stoppage time
Serie A: Frank Ribery scores in Fiorentina's 3-1 victory over AC Milan; Napoli get back to winning ways with 2-1 triumph over Brescia
Napoli got back to winning ways with a 2-1 victory against Brescia on Sunday, whereas Franck Ribery sealed a 3-1 win for Fiorentina against ten-man AC Milan that pushed the northerners down to 16th in the Serie A table.
Serie A: FIFA president Gianni Infantino calls for action after racist abuse halts match between Atalanta and Fiorentina
FIFA president Gianni Infantino said on Sunday the problem of racism in Italian football has "not improved" after a Serie A match between Atalanta and Fiorentina was halted for several minutes because of racist abuse of Brazilian Dalbert.
Coppa Italia: Defending champions Juventus knocked out by Duvan Zapata-led Atalanta; Fiorentina thrash AS Roma 7-1
Four-time defending champions Juventus crashed out of the Coppa Italia with a defeat by Atalanta, as AS Roma suffered a humiliating 7-1 thrashing at the hands of Fiorentina.
Coppa Italia: Inter Milan thrash Benevento 6-2 in closed-door match to breeze into quarter-finals; Napoli beat Sassuolo
Inter ran riot in a San Siro left empty as part of the punishment the club received for the monkey chants directed at Koulibaly during their win over Napoli.
Serie A: Mario Mandzukic's lone goal helps Juventus pile on misery on Roma; AC Milan drop out of top four
Mario Mandzukic scored the only goal for Juventus to dump further hurt on crisis club Roma and keep the champions unbeaten and on track for an eighth straight Serie A title.
UEFA Nations League: Italy coach Roberto Mancini says team lacks confidence after draw against Poland
Roberto Mancini, 58, was appointed after the four-time winners Italy's shock failure to qualify for the 2018 FIFA World Cup.
Fiorentina had been frustrated in Sardinia before Babacar came on after 70 minutes and picked up a Federico Chiesa cross with eight minutes to go.