Werder Bremen, who finished third from bottom in the top division, will have to do without captain Niklas Moisander for the second leg on Monday after he was sent off late with his second yellow card for a poor challenge.
Borussia Monchangladbach secured the fourth spot ahead of Bayer Leverkusen on the final day of the Bundesliga season with a 2-1 win over Hertha Berlin.
Bundesliga: Robert Lewandowski breaks goalscoring record; Erling Haaland brace helps Dortmund secure second place
It means Dortmund are assured of second place with one game of the season remaining. Despite defeat, Leipzig are virtually assured of Champions League football next season.
Bundesliga: Bayern Munich coach Hansi Flick warns relegation-threatened Werder Bremen against expecting any ‘gifts'
Werder Bremen are on the brink of relegation but they should not expect any rescue gifts from Bayern Munich when they meet on Tuesday with the Bavarians eyeing their eighth consecutive league title, Bayern coach Hansi Flick said on Monday.
Werder Bremen have spent only one season outside the Bundesliga since it was launched in 1963 and while their run of 13 home matches without a win has left them deep in relegation trouble, club officials are not throwing in the towel.
Bundesliga: Schalke's winless run continues in draw against Union Berlin; Wolfsburg beat Werder Bremen
They were heading for a largely uneventful 0-0 draw in torrential rain before the 82nd minute, when Wolfsburg's substitute winger Felix Klaus curled in a cross from the right and Wout Weghorst met it with a bouncing header into the Werder goal.
Bundesliga: David Wagner's tenure with Schalke on last leg after shock defeat to relegation-threatened Werder Bremen
Just 10 days ago, club bosses were moved to guarantee Wagner, whose contract runs out in 2022, would still be in charge next season but last week’s 1-0 home loss to relegation-threatened Werder Bremen has ratcheted up the pressure.
Bundesliga: Stefan Ilsanker's brace fires Eintracht Frankfurt to comfortable win as Werder Bremen remain in drop zone
Eintracht climbed to 11th position in the Bundesliga table with 35 points after the win over Bremen.
Bundesliga: Bayern Munich inch closer to eighth straight title with thrashing of Fortuna Dusseldorf; Hertha Berlin beat Augsburg
Robert Lewandowski scored twice as Bayern Munich took a confident step closer to an eighth straight German title with a 5-0 demolition of Fortuna Dusseldorf in the Bundesliga.
Bundesliga talking points: From Bayern Munich's eight-game winning run to Kai Havertz's goal-scoring form
Quick, smart and highly-skilled, Havertz scored twice for the second consecutive game on Sunday as top European clubs are closing in.
Bundesliga: Raphael Guerreiro, Achraf Hakimi score as Dortmund beat Wolfsburg; Kai Havertz bags brace in Bayer win
The result lifted Dortmund to 57 points, one behind Bayern, who are in action against Eintracht Frankfurt later on Saturday. The Bundesliga resumed last week with no fans, after more than two months of suspension due to the COVID-19 pandemic.
Bundesliga: Bayer Leverkusen coach Peter Bosz says build-up to league's resumption has been 'spooky'
Preparing for a resumption of the Bundesliga, the first major sports league to return to action, has meant a multitude of health measures that have left the Dutch coach with an eerie feeling
Bundesliga: Erling Haaland scores yet again as Borussia Dortmund beat Werder Bremen; RB Leipzig win big against Schalke
Erling Haaland scored yet again to help Borussia Dortmund beat Werder Bremen 2-0, and RB Leipzig followed by routing Schalke 5-0 to keep the pressure on Bundesliga leader Bayern Munich.
Bundesliga: Borussia Dortmund hammer Eintracht Frankfurt 4-0, gain momentum ahead of Champions League clash against PSG
Dortmund bounced back from two consecutive defeats in the German Cup and league last week, filling up on confidence ahead of Tuesday’s Champions League Round of 16 first leg at home to PSG
DFB Pokal: Borussia Dortmund knocked out by Bundesliga strugglers Werder Bremen; RB Leipzig lose to Eintracht Frankfurt
Dortmund lost 3-2 to Werder Bremen, a major upset given that Werder are battling against relegation from the Bundesliga while Dortmund challenges for the title
Bundesliga: Bayer Leverkusen beat Fortuna Dusseldorf to move up to fifth place, Werder Bremen still in danger
Bayer Leverkusen moved up to fifth place in the Bundesliga with a 3-0 win over bottom side Fortuna Dusseldorf, while Werder Bremen was unable to escape the relegation zone on Sunday.
Wolfsburg’s Maximilian Arnold fired in a stoppage time winner as they beat Borussia Moenchengladbach 2-1 and sent them dropping into second place in the Bundesliga after leading the title race for the last two months.
Bundesliga: Bayern Munich's Philippe Coutinho scores hat-trick in 6-1 rout of Werder Bremen; Borussia Dortmund win against Mainz
Bayern Munich's man-of-the-match Coutinho, who also helped set up two more goals, delivered at just the right time with the Bavarians, struggling to find their rhythm under interim coach Hansi Flick
Bundesliga: Sebastian Andersson's double helps Union Berlin defeat Cologne; Paderborn beat Werder Bremen
Union is seven points clear of the relegation zone after 14 games, and closer in points to defending champion Bayern Munich than to teams struggling at the bottom.
Bundesliga: Patrick Herrmann's brace powers Borussia Monchengladbach to emphatic victory over Werder Bremen, extend stay at top
Borussia Monchengladbach maintained their four-point lead at the top of the Bundesliga on Sunday as Patrick Herrmann scored twice in an emphatic 3-1 home win over Werder Bremen.