Jennifer Brady is the last player standing among those forced into a hard 14-day quarantine after arriving in Australia.
Australian Open 2021: Semi-final loss compounds Serena's four years of hurt as No 24 continues to elude
Here is a rundown of the American great's failed attempts at history since winning her last major at the 2017 Australian Open while pregnant.
Australian Open 2021: 'I'm done', Serena Williams in tears after falling short of 24th Grand Slam yet again
Pressed on what caused her to make so many mistakes, tears began to flow as she told reporters: "I don't know. I'm done," before walking out.
Australian Open 2021: Dominant Naomi Osaka ends Serena Williams’ bid for 24th Slam with straight sets win
Williams, who won her last major in Melbourne in 2017, was left stranded on 23 Grand Slam titles as she strives to match Margaret Court's all-time record.
Australian Open 2021 Highlights: Novak Djokovic through to ninth final; Jennifer Brady sets up Naomi Osaka clash
Australian Open 2021 Semi finals: Novak Djokovic will look to stay on track for an 18th Grand Slam title, while Serena Williams has the formidable figure of Naomi Osaka blocking her path to a record-equalling 24th when the Australian Open semi-finals begin.
Australian Open 2021: Serena Williams says she must raise her game against 'cool cat' Naomi Osaka in semi-final
Williams, 39, continues to chase Margaret Court's elusive all-time mark of 24 major titles, after losing four Slam finals since her 2017 victory at Melbourne Park while pregnant.
Australian Open 2021: Serena Williams to face 'incredible' Naomi Osaka after statement win over Simona Halep
The 39-year-old, 23-time major winner has lost four Slam finals while chasing Margaret Court's all-time mark since her last major victory at Melbourne Park in 2017 while pregnant.
Australian Open 2021: Novak Djokovic beats Alexander Zverev, into ninth semi-final; Serena Williams sets up Naomi Osaka last-four clash
Djokovic pulled himself together and pulled out the victory, reaching his ninth semi-final at Melbourne Park by eliminating No 6 seed Zverev 6-7 (6), 6-2, 6-4, 7-6 (6)
Australian Open 2021: Serena Williams against Simona Halep, Naomi Osaka versus Hsieh Su-wei in quarter-finals
Williams leads the series 9-2, but Halep won their most recent showdown, 6-2, 6-2 in the 2019 Wimbledon final.
Australian Open wins over Tsvetana Pironkova, eighth seed Bianca Andreescu, Sara Errani and Marketa Vondrousova mean that Hsieh Su-wei will face Naomi Osaka, 12 years her junior, on Rod Laver Arena on Tuesday for a place in the semi-finals.
Australian Open 2021: 'Very stressed' Naomi Osaka saves match points to set up all-Asian quarterfinal
Naomi Osaka, the third seed, was on the verge of elimination at 3-5, 15-40 on her serve in the third set before rattling off four points in a row.
Australian Open 2021: Dominic Thiem stunned by Grigor Dimitrov; Serena Williams, Naomi Osaka survive
A fatigued-looking Thiem, coming off a five-set win over Australia's Nick Kyrgios, slumped in straight sets to Bulgaria's Grigor Dimitrov who took it 6-4, 6-4, 6-0 in just over two hours.
The Spanish 14th seed, the losing finalist last year, was on the cusp of making the quarter-finals once more at 5-3, 40-15 in the third set.
Australian Open 2021 to continue without fans, players to compete in biosecure bubble as Melbourne goes into lockdown
Premier Daniel Andrews of Victoria state, which includes Melbourne, said the tennis venue would be considered a "workplace" that can continue to function with limited staff.
Three-time Grand Slam winner Naomi Osaka had an uneasy feeling on Wednesday before her Round 2 match against Caroline Garcia after waking up in a panic after a nightmare.
It was an effortless performance from US Open champion Osaka, who revealed she was battling nerves ahead of the year's opening Grand Slam.
Australian Open 2021: Venus Williams hailed by sister Serena, Naomi Osaka after winning opening round
Williams, who played her first Australian Open in 1998, battled past fellow veteran Kirsten Flipkens 7-5, 6-2 on Margaret Court Arena in her 88th Grand Slam to ensure she lived to fight another day.
Australian Open 2021: Serena Williams, Naomi Osaka sail into second round; Angelique Kerber, Gael Monfils out at first hurdle
Venus Williams, on Monday, won a Grand Slam match for the first time since 2019.
With the Grand Slam being played amid the COVID-19 pandemic, the organisers will be under as much pressure as the players to perform and complete the event.
Australian Open 2021 women's singles preview: Ashleigh Barty gets decent draw; Naomi Osaka gunning for second AO title
Ahead of the Australian Open's long-awaited return, Firstpost takes a look at the women's singles draw and attempts to determine the potential outcome of all four quarters