Valencia trail Barcelona by four points at the top of the table as the Catalans have amassed 34 points from a possible 36.
La Liga: Real Madrid's Gareth Bale returns to training after two-month long injury ahead of Malaga clash
Gareth Bale finally returned to training with his Real Madrid team-mates on Friday and will be eased back in after two months on the sidelines, according to coach Zinedine Zidane.
La Liga: Valencia's record-breaking win keeps them in hunt for top spot; Athletic Bilbao end losing streak
A club record eighth straight La Liga win for Valencia puts them six points clear of Real Madrid in third and sets up a mouthwatering top of the table showdown at home to Barcelona next Sunday.
Real Madrid captain Sergio Ramos had his nose broken in a bad-tempered Madrid derby at Atletico Madrid, tests confirmed on Sunday
Isco was given a standing ovation as he limped off 25 minutes from time in Saturday's 5-0 thrashing of Costa Rica after a reckless challenge from Kendall Waston.
Andres Iniesta, Jordi Alba and Alvaro Morata also netted in a one-sided display from the 2010 World Champions Spain to win 5-0.
La Liga: Barcelona stride past Malaga to consolidate top spot amidst Catalan chaos; Valencia thrash Sevilla
Even as 4,50,000 independence supporters piled onto the streets of Barcelona, it was business as usual for Barca barring the usual cries in favour of independence in the 17th minute and sporadic chants of "freedom".
On the field, Barcelona have made light of all manner of distractions this season to perform far better than expected.
La Liga: Lacklustre Atletico Madrid held by Leganes, Sevilla hand Malaga their sixth defeat of season
Atletico Madrid's poor week following their Champions League defeat by Chelsea continued as they lost more ground in the La Liga title race in a 0-0 draw at Leganes.
"That Messi scored four goals now isn't news. He is extraordinary. I have run out of words for him," said Barcelona boss Ernesto Valverde after the Argentine's 43rd career hat-trick.
Antoine Griezmann ensured Malaga didn't spoil Atletico Madrid's moving in party at their new 68,000 Wanda Metropolitano stadium with the only goal in a 1-0 win on Saturday.
Levante celebrated being back in Spanish top-flight football with a narrow 1-0 victory against regional rivals Villarreal.
Neymar to PSG: From Champions League highs to lows of tax fraud charges, star striker's tryst with Barcelona
Neymar's anticipated world record transfer move to Paris Saint-Germain will bring to an end an epic four-year spell at Barcelona.
Against Juventus, Isco will seek a repeat performance of the semifinal against Atletico Madrid to crown Real Madrid European champions yet again.
"It is the happiest day of my professional life by a distance. Right now I would like to stand up here and dance," Zidane added in a press conference.
A look at the games which played a pivotal role in Real Madrid ending their five-year wait to win La Liga title.
Despite the joy of helping seal Real Madrid's 33rd La Liga title on Sunday, Cristiano Ronaldo slammed his treatment by the media, saying he is not "the devil" he is made out to be.
La Liga: Real Madrid a point away from winning title and ending Barcelona's reign as Spanish champions
Real Madrid can only throw away a first La Liga title in five years on Sunday with a shock defeat at Malaga as they look set to depose rivals Barcelona's run as Spanish champions.
Zinedine Zidane hailed Real Madrid's remarkable strength in depth as the key to his side entering their final game of the season at Malaga on the brink of lifting their first La Liga title since 2012.
Argentine defender Martin Demichelis, who won one Premier League and four Bundesliga titles, says he will retire after the season.