Pioli, 55, and his assistant Giacomo Murelli, both tested positive mid-November and had been in isolation.
A minute's silence had been observed before the game, as in all matches in Italy, in memory of Maradona who spent seven years at Napoli.
Serie A: Ageless Ibrahimovic's brace helps AC Milan beat Napoli; Inter Milan come from behind to defeat Torino
Zlatan Ibrahimovic scored twice to raise his total to 10 goals in six games played as Milan won 3-1 at Napoli on Sunday.
Fiorentina have won only two of their seven league matches and are 12th in Serie A, nine points behind leader AC Milan.
Serie A: Joao Pedro matches Italy legend Riva's 52-year Cagliari record, M'Bala Nzola brace lifts Spezia
Sampdoria and Cagliari are 10th and 11th respectively, both on 10 points after seven games, as they push for European football next season.
Romelu Lukaku and Danilo D'Ambrosio scored late to help Inter Milan get their season off to a winning start.
Serie A: Gaetano Castrovilli's lone goal headlines Fiorentina's win over Torino; Roma held by Hellas Verona
Bosnia forward Edin Dzeko was left on the bench for the side from the capital amid reports he is set for a move to Juventus.
The coronavirus-delayed Serie A season ended almost 12 months after it started on 24 August, 2019.
Serie A: Inter Milan's draw against Fiorentina takes Juventus one step closer to title; Parma beat Napoli
Romelu Lukaku and Alexis Sánchez each hit the woodwork for Inter, who also had a potential goal from Antonio Candreva waved off for offside.
Lazio's Luis Alberto struck seven minutes from time against Fiorentina to cut the gap to league leader Juventus back to four points.
Coronavirus Outbreak: Serie A remains on hold as Italian government examines medical guidelines before announcing resumption
Serie A teams have been allowed to start practice this week but only with players training individually and respecting social distancing. Full team practice is due to begin on 18 May, but only if the medical protocol is approved.
Coronavirus Outbreak: Three Fiorentina players have tested positive for COVID-19, confirm Serie A club
Three players at Serie A football club Fiorentina have tested positive for COVID-19 as well as three members of the back-up staff, the Florence-based club said in a statement on Thursday.
Coronavirus Outbreak: Serie A team Cagliari's staff agree to go without pay for one month, says president Tommaso Giulini
Serie A, like football leagues around the world, has been brought to a standstill and there is no indication of when it may be able to start again, although the Italian federation says it wants to finish the season
Serie A: Italian league confirms five matches will be played behind closed doors due to coronavirus outbreak in country
Italy’s Serie A football league has confirmed that five matches will be played behind closed doors at the weekend because of the coronavirus outbreak in the country, including the clash between leaders Juventus and third-placed Inter Milan on Sunday.
Serie A: Ronaldo matches record in 1,000th game as Juventus beats Spal; Fiorentina secure draw against AC Milan
Ronaldo scored for an 11th straight league game, matching the Serie A record set by Gabriel Batistuta in 1994 and equaled by Fabio Quagliarella last season.
Serie A: Ronaldo extends scoring streak as Juventus thump Fiorentina; Romelu Lukaku's brace brings Inter Milan back on track
Cristiano Ronaldo converted two penalties to tie a club record by scoring for a ninth straight game as Serie A leader Juventus bounced back from a rare loss with a 3-0 victory over Fiorentina
Coppa Italia: Nicolo Barella's stunning volley sends Inter Milan to semi-final with narrow victory over Fiorentina
Barella scored what was to prove the winner in the 67th minute, volleying in from 20 yards after Fiorentina defender Nikola Milenković headed out a cross.
Serie A: Ciro Immobile's treble helps Lazio crush Sampdoria; Dusan Vlahovic's stunner guides Fiorentina to win over Napoli
Ciro Immobile scored a hat trick on Saturday to help Lazio crush Sampdoria 5-1 as Simone Inzaghi’s side continued to look like a genuine title contender.
Coppa Italia: Paulo Dybala scores brace in Juventus win; 10-man Fiorentina beat Atlanta to set up Inter Milan clash
Dybala and Gonzalo Higuaín combined for the opening goal in the 16th minute, exchanging a series of passes before Higuaín finished it off
Coppa Italia: Romelu Lukaku's brace sees Inter Milan beat Cagliari; Holders Lazio to face Napoli in quarter-finals
Antonio Conte’s Inter Milan side dominated from the start and took the lead after just 22 seconds when Lukaku capitalized on an error from Cagliari midfielder Nahitan Nandez