Coach Andrea Pirlo said Ronaldo, the top scorer in Serie A this season with 25 goals, would rest to avoid risking a more serious injury.
Antonio Conte's side are on course for a 19th Serie A title and first since 2010.
A fuming Ronaldo received a yellow card for his protests and at the final whistle, as he stormed from the pitch, he threw his captain's armband to the ground.
Serie A: Cristiano Ronaldo shifts focus as Juventus aim to reboot title defence against city-rivals Torino
Juventus are third, 10 points adrift of leaders Inter Milan, with both teams having 11 games left to play this season.
Serie A giants Juventus reimagined their look. French heavyweights PSG partnered with Jordan Brand. Now Arsenal and Inter Milan are following suit. But football’s interest in design has little to do with the sport itself.
FIFA World Cup 2022 Qualifiers: Cristiano Ronaldo scores as Portugal beat Luxembourg; Belgium, Dutch score big wins
Juventus forward Ronaldo bagged his 103rd international goal five minutes after the break to stop his side falling foul of a Luxembourg team whose recent performances have defied their small status and tiny population.
Cristiano Ronaldo's discarded Portugal armband up for auction to help collect money for six-month-old baby's surgery
While heading straight into the dressing room before the final whistle, Ronaldo angrily threw down his captain armband near the touchline.
FIFA World Cup qualifiers: Coronavirus pandemic blamed for lack of VAR after Cristiano Ronaldo's complaints
Portugal's path to the 2022 World Cup has been complicated by Ronaldo being denied a stoppage-time winner against Serbia on Saturday
FIFA World Cup 2022 Qualifiers: Portugal coach Fernando Santos says 'not possible' Cristiano Ronaldo goal ruled out
Without VAR or goalline technology in use, officials decided that Serbia defender Stefan Mitrovic had cleared even though images appeared to show the ball had crossed the line.
Portugal drew 2-2 in Belgrade, despite Ronaldo believing he had scored the winner in the third minute of stoppage time when the ball appeared to cross the goalline before Stefan Mitrovic cleared.
FIFA World Cup 2022 Qualifiers, matchday: Netherlands hope to rebound after loss, Portugal visit Serbia
The Netherlands look to get their campaign back on track against Latvia after slumping to a 4-2 defeat to Turkey in their first Group G match.
Ronaldo has been under fire in Italy after Juve's Champions League last-16 exit to Porto and the weekend defeat by Benevento which left them 10 points behind Serie A leaders Inter Milan.
Argentine forward Adolfo Gaich scored the only goal after 69 minutes following a bad pass across the penalty area from Juventus midfielder Arthur.
Reports in Spain have suggested Ronaldo is keen on returning to Real Madrid, where he enjoyed a hugely successful nine years before leaving in 2018 for 100 million euros.
Ronaldo's goals include 668 for club sides - five with Sporting Lisbon, 118 for Manchester United, 450 for Real Madrid and 95 for Juventus. He has also scored 120 for Portugal.
Serie A: Cristiano Ronaldo hits back with hat-trick for Juventus, Inter Milan move nine points clear
Defending champions Juventus are 10 points behind leaders Inter Milan, who beat Torino 2-1 earlier Sunday, but with a game in hand.
‘True champions never break’: Cristiano Ronaldo urges Juventus to move on from Champions League exit
The Juventus star spoke for the first time since the Serie A champions crashed out of the UEFA Champions League after a Round of 16 loss to Porto
In 16 seasons with Madrid, Ramos has helped the club win four Champions League titles, five Spanish leagues and two Copa del Reys, with both his hardnose defending and some timely goals.
Champions League: Fabio Capello questions Juventus boss Andrea Pirlo over Cristiano Ronaldo's error against Porto
Juventus won the game 3-2 but was eliminated on away goals after Tuesday's match ended 4-4 on aggregate.
Champions League: Sergio Oliveira's extra-time goal helps Porto seal quarter-final spot despite Juventus win
Sergio Oliveira's extra-time free-kick sent twice former winners Porto through to the quarter-finals as the tie finished 4-4 on aggregate.