Blaise Matuidi played 98 games for Juventus in Serie A and 133 times in all competitions in his three years at the club, second only to Argentine forward Paulo Dybala (134).
Cristiano Ronaldo reported to Juventus’ training center for medical tests on Tuesday after a 10-week absence.
Rugani and Matuidi are two of three Juventus players who last month tested positive for the virus. The duo were asymptomatic, but the third player, forward Paulo Dybala, showed strong symptoms.
Dybala the third player at the club, one of the biggest in Europe and Italy’s most successful in domestic terms, to test positive after midfielder Blaise Matuidi, a World Cup winner with France in 2018, and defender Daniele Rugani.
Euro 2020 Qualifiers: Juventus midfielder Blaise Matuidi ruled out of France squad with rib injury, replaced by Matteo Guendouzi
Matuidi picked up the knock in the 1-0 win over AC Milan that kept champions Juve top of Serie A, and failed to make the trip to Paris ahead of the two Group H clashes on Thursday and Sunday which could seal qualification for next summer's finals
Both goals came from penalties with Dybala's strike from the spot after 50 minutes cancelled out by Lecce's Marco Mancosu's effort six minutes later.
Serie A: AS Roma bans fan for life, files police complaint following racist abuse aimed at Juan Jesus
Roma said on the club's official Twitter account it had filed a police complaint against the fan for using social media to send "disgusting racist insults" to the player.
Gianni Infantino makes plea to 'kick racism out' of Italian football during FIFA Best Football Awards ceremony in Milan
FIFA president Gianni Infantino on Monday made a plea to "kick racism out" of Italian football, during the FIFA Best Football Awards ceremony in Milan.
Atletico and Juve have a point each while Lokomotiv Moscow top Group D after beating Bayer Leverkusen 2-1 away from home
Champions League: After Neymar's botched transfer and arrival of Mauro Icardi, can Paris-Saint Germain find glory in Europe?
When Real Madrid and Paris-Saint Germain last met in Europe two seasons ago, a PSG side missing an injured Neymar were dumped out in the last 16.
Inter Milan's fan group 'L'Urlo della Nord' told Lukaku in an open letter that he was seeing racism where there was none.
Serie A: Massimiliano Allegri parts ways with champions Juventus amidst reports of fall-out with club directors
Despite signing Cristiano Ronaldo from Real Madrid last summer, Allegri and the Bianconeri failed to make it past the quarter-finals of the European football's top competition for the second successive season
Champions League: Serie A leaders Juventus put domestic responsibilities on back-burner to focus efforts on youthful Ajax side
Juventus have put their bid to wrap up an eighth consecutive Serie A title on the back burner as they focus on their European ambitions
Ligue 1: Amiens captain Prince Gouano halts match against Dijon to confront fans after alleged racial abuse
Friday's French Ligue 1 game between Dijon and Amiens was temporarily suspended after the visitors' captain Prince Gouano complained he was racially abused.
Serie A needs to act hard against racism after unacceptable abuse of Juventus' Moise Kean, says Italy boss Roberto Mancini
Italy coach Roberto Mancini called for tough measures in Serie A after the "unacceptable" racist abuse of Juventus's Italian international Moise Kean in Cagliari.
Serie A: Leonardo Bonucci condemns all forms of racism, says his comments after Juventus game were 'misunderstood'
Under-fire Juventus centre-back Leonardo Bonucci said Wednesday he had been "too hasty" when blaming young teammate Moise Kean for being partly responsible for racist insults hurled at him in a Serie A game at Cagliari.
Yaya Toure, Raheem Sterling slam Leonardo Bonucci, Massimiliano Allegri's response to racist abuse of Moise Kean
Yaya Toure and Raheem Sterling slammed Massimiliano Allegri and Leonardo Bonucci after they said Moise Kean shared the blame for racist abuse by Cagliari fans.
Serie A: Racist chants mar Juventus’ win at Cagliari as Moise Kean continues goal-scoring spree; Udinese hold AC Milan
Rising Italy star Moise Kean was targeted by racist abuse after he continued his goal-scoring spree to push Juventus closer to an eighth consecutive Serie A title on Tuesday with a 2-0 win over Cagliari.
Serie A: Inter Milan to play two matches behind close doors as punishment for racist chants towards Kalidou Koulibaly
Kalidou Koulibaly was subjected to monkey noises from the crowd several times during Napoli's match against Inter Milan at the San Siro.
Serie A: Juventus extend perfect start to title defence to six games with win over Bologna; AS Roma beat promoted Frosinone
Paulo Dybala and Blaise Matuidi scored early as Juventus extended their perfect start to their Serie A title defence to six games on Wednesday with a 2-0 win over Bologna, as Roma ended their losing streak with a 4-0 success over promoted Frosinone.